| 中文名称Chinese name
|
MERS-CoV EMC/2012滴鼻感染DPP4-KI转基因小鼠模型 |
| 编号Model number |
|
| 英文名称English name |
MERS-CoV EMC/2012 intranasally infection trangenic DPP4-KI mouse model |
| 物种Species name |
小鼠
|
| 品系Strain name |
|
| 人类疾病Human diseases |
MERS |
| 传染性Infectivity |
是 |
| 微生物Microorganism |
|
| 类别Category |
MERS-CoV感染动物模型 |
| 造模因素Modeling factors |
|
| 制作方法Making method |
Using a plasmid-mediated gene targeting strategy, a congenic C57BL/6 mouse was developed by replacing mouse Dpp4 exons 10–12 with the corresponding human DPP4 codons (hDPP4-KI). For the studies reported, we used mice homozygous for hDPP4.A congenic C57BL/6 mouse with mouse Dpp4 exons 10–12 replaced with the human DPP4 codons was generated by Taconic Biosciences.This change in exon
coding sequence allowed the modified mouse gene product to display amino acids required for virus binding (18–20). The targeting strategy was based on National Center for Biotechnology Information transcripts NM_010074_3 (mouse) and NM_001935_3 (human). The targeting vector was generated using BAC clones from the mouse C57BL/6J RPCIB-731 and human RPCIB-753 BAC libraries. Mouse genomic sequence from codon I264 in exon 10 to codon V340 in exon 12 was replaced with its human counterpart.. Human DPP4 KI mice were anesthetized with ketamine/xylazine (87.5 mg/kg ketamine/12.5 mg/kg xylazine) and infected intranasally with MERS-CoV in 50 μL DMEM. |
| 生物学特征Biological characteristics |
|
| 医学用途Medical use |
用于MERS致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation |
|
| 病理表现Pathology |
|
| 发病天数Days of onset |
|
| 发病过程Pathogenesis process |
|
| 临床诊断Clinical diagnosis |
|
| 评价模型成功的指标Indicators for evaluating model success |
|
| 保藏单位Preservation unit |
爱荷华大学@@https://uiowa.edu/ |
| 参考文献Reference |
Mouse-adapted MERS coronavirus causes lethal lung disease in human DPP4 knockin mice.,PMID:28348219,DOI:10.1038/nmicrobiol.2016.286 |
| 录入/更新时间Date |
2021.7.20 |
| 备注Remarks |
|
当前位置：
