| 中文名称Chinese name
|
SARS-CoV Urbani 气管滴鼻联合感染雪貂模型 |
| 编号Model number |
|
| 英文名称English name |
SARS-CoV Urbani intratracheally and intranasally infected ferret model |
| 物种Species name |
雪貂
|
| 品系Strain name |
雪貂
|
| 人类疾病Human diseases |
SARS |
| 传染性Infectivity |
是 |
| 微生物Microorganism |
|
| 类别Category |
SARS-CoV感染动物模型 |
| 造模因素Modeling factors |
|
| 制作方法Making method |
To generate the vaccine, 5.94 mL of virus stock ( 1*10^5 TCID50/mL)was treated with 60 mL of 3.7% formalin for 48 h at 37 °C. The inactivated virus (6 mL) was then dialyzed in 2 L of PBS at room temperature for 48h. Medium from uninfected Vero cells was treated in the same way to prepare mock vaccine.Vaccine was plated on Vero cells for 7 days to confirm inactivation. Vaccination was done intramuscularly at 0.5 mL per leg without adjuvant. The virus challenge ( 1x10^5 TCID50/mL) was split into 2 parts: 0.25 mL was given intratracheally, and 0.25 mL was given intranasally. A laryngoscope was used for the intratracheal portion, which was delivered via a 1-cc syringe with a 22-gauge plastic angiocatheter attached. The intranasal inoculum was delivered to each nostril via a plastic bulb–type pipettor. |
| 生物学特征Biological characteristics |
|
| 医学用途Medical use |
用于SARS-CoV致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation |
|
| 病理表现Pathology |
|
| 发病天数Days of onset |
|
| 发病过程Pathogenesis process |
|
| 临床诊断Clinical diagnosis |
|
| 评价模型成功的指标Indicators for evaluating model success |
|
| 保藏单位Preservation unit |
美国食品药品管理局@@https://www.fda.gov |
| 参考文献Reference |
Severe acute respiratory syndrome coronavirus infection in vaccinated ferrets.,PMID:17922397,DOI:10.1086/522431 |
| 录入/更新时间Date |
2021.7.20 |
| 备注Remarks |
|
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