| 中文名称Chinese name
|
HIV-1 HIV-1ADA腹腔途径感染TK-NOG小鼠模型 |
| 编号Model number |
|
| 英文名称English name |
HIV-1 HIV-1ADA intraperitoneally infected TK-NOG mouse model |
| 物种Species name |
小鼠
|
| 品系Strain name |
|
| 人类疾病Human diseases |
HIV |
| 传染性Infectivity |
是 |
| 微生物Microorganism |
|
| 类别Category |
HIV感染动物模型 |
| 造模因素Modeling factors |
|
| 制作方法Making method |
TK-NOG strain colony by breeding female TK-NOG mice with male non-TK-NOG littermates and select transgenic offspring by genotyping. Inject the mice with 1 x 10^3 to 1 x 10^4 tissue culture infectious doses 50 (TCID50) HIV-1ADA in a volume of 100 - 200 µL per mouse, intraperitoneally.The mice are infected (n = 10) with HIV at 4 - 5 months posttransplantation and sacrificed 5 weeks postinfection.draw two to three drops (~100 µL) of blood from the submandibular vein by pricking it with a 5 mm lancet, and isolate the serum by centrifuging (1,500 x g for 10 min at 4 °C) for the alanine aminotransferase (ALT) assay to assess the degree of liver damage. |
| 生物学特征Biological characteristics |
|
| 医学用途Medical use |
用于HIV致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation |
|
| 病理表现Pathology |
|
| 发病天数Days of onset |
|
| 发病过程Pathogenesis process |
|
| 临床诊断Clinical diagnosis |
|
| 评价模型成功的指标Indicators for evaluating model success |
|
| 保藏单位Preservation unit |
內布拉斯加大学医学中心@@https://www.unmc.edu/ |
| 参考文献Reference |
Establishment of the Dual Humanized TK-NOG Mouse Model for HIVassociated Liver Pathogenesis,PMID:31566621,DOI:10.3791/58645 |
| 录入/更新时间Date |
2021.7.20 |
| 备注Remarks |
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