| 中文名称Chinese name
|
HIV-1 NL4-3-HA 腹腔途径感染NOD小鼠模型 |
| 编号Model number |
|
| 英文名称English name |
HIV-1 NL4-3-HA intraperitoneally infected NOD mouse model |
| 物种Species name |
小鼠
|
| 品系Strain name |
NOD小鼠
|
| 人类疾病Human diseases |
HIV |
| 传染性Infectivity |
是 |
| 微生物Microorganism |
|
| 类别Category |
HIV感染动物模型 |
| 造模因素Modeling factors |
|
| 制作方法Making method |
NOD.Cg-Prkcdscid Il2rgtm1Wjl/SzJ (NSG) neonatal mice were sub-lethally irradiated and injected with 1 x 10^6 human fetal liver CD34+ cells per mouse. Nine humanized mice were infected with the HIV-1 strain NL4-3-HA (47) for 10 weeks, and then 4 were switched to ART-containing feed. Humanized mice between ages 12-20 weeks, engrafted with at least 30% human CD45+ cells in blood, of which at least 10% were CD4+, were infected via intraperitoneal injections containing 5-10 x 10^5 infectious units of HIV-1. This formulation was calculated by consideration of the average amount of food consumed by the mice (3.5g per day) and their average weight (20g) and provided average daily drug doses of Emtricitabine (FTC) at 87.5mg/kg/day, Tenofovir (TDF) at 131mg/kg/day, and Raltegravir (RAL) and Darunavir (DRV) at 175mg/kg/day each. |
| 生物学特征Biological characteristics |
|
| 医学用途Medical use |
用于HIV致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation |
|
| 病理表现Pathology |
|
| 发病天数Days of onset |
|
| 发病过程Pathogenesis process |
|
| 临床诊断Clinical diagnosis |
病毒在血液中复制 |
| 评价模型成功的指标Indicators for evaluating model success |
|
| 保藏单位Preservation unit |
南加州大学@@https://www.usc.edu/ |
| 参考文献Reference |
Humanized Mouse Model of HIV-1 Latency with Enrichment of Latent Virus in PD-1 + and TIGIT + CD4 T Cells,PMID:30842333,DOI:10.1128/JVI.02086-18 |
| 录入/更新时间Date |
2021.7.20 |
| 备注Remarks |
|
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