| 制作方法Making method |
For the acute and reactivation models, inject 6–8 week-old NS γ-chainnull mice intraperitoneally with human PBMCs derived from a healthy donor or HIV-infected patient who was under ART,respectively. In both models, include mice injected with PBMCs from a healthy donor without HIV infection (sham) as controls.Mice were clean the mice with a pre-wetted alcohol pad in the lower left/right quadrant of the abdomen. Inject 15,000 TCID50 of the HIV BaL virus contained in 200 µL of sterile RPMI 1640.Administer oral ART at least 3 weeks after infection, when high viral load is observed, in the chronic, acute, and reactivation models. Calculate the doses of tenofovir disoproxil fumarate (TDF), emtricitabine (FTC), and raltegravir (RAL), according to Km values of 37 and 3 for humans and mice, respectively26. Typically, the human-equivalent doses of TDF, FTC, and RAL are 61.7 mg/kg/day, 40.7 mg/kg/day, and 164 mg/kg/day, respectively. |
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