当前位置：
> SARS-CoV 滴鼻感染BALB/c小鼠模型
| 中文名称Chinese name | SARS-CoV 滴鼻感染BALB/c小鼠模型 |
|---|---|
| 编号Model number | |
| 英文名称English name | SARS-CoV intranasally infection of BALB/c mouse model |
| 物种Species name | 小鼠 |
| 品系Strain name | BALB/c小鼠 |
| 人类疾病Human diseases | SARS |
| 传染性Infectivity | 是 |
| 微生物Microorganism | |
| 类别Category | SARS-CoV感染动物模型 |
| 造模因素Modeling factors | |
| 制作方法Making method | BALB/c mice (female, 6-week-old (Japan SLC, Shizuoka, Japan); n = 6–7; total, 25) were immunized subcutaneously twice at 2 week intervals with 1 µg, 0.5 µg, 0.1 µg, or 0.05 µg doses of the protein in 100 µL PBS.Approximately 3 weeks after the second immunization, mice were anesthetized via intraperitoneal injection of a mixture of 1.0 mg ketamine (Daiichi Sankyo Company, Tokyo, Japan) and 0.02 mg xylazine (Byer Japan, Osaka, Japan) (0.08 ml/10 g of body weight). These mice were then inoculated intranasally with SARS-CoV (106 TCID50 in 30 µl of 2% FBS-MEM). |
| 生物学特征Biological characteristics | |
| 医学用途Medical use | 用于SARS-CoV致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation | |
| 病理表现Pathology | |
| 发病天数Days of onset | |
| 发病过程Pathogenesis process | |
| 临床诊断Clinical diagnosis | 血管周围水肿，炎症细胞浸润 |
| 评价模型成功的指标Indicators for evaluating model success | |
| 保藏单位Preservation unit | 日本国立传染病研究所@@https://www.niid.go.jp/ |
| 参考文献Reference | Gold nanoparticle-adjuvanted S protein induces a strong antigen-specific IgG response against severe acute respiratory syndrome-related coronavirus infection, but fails to induce protective antibodies and limit eosinophilic infiltration in lungs.,PMID:31692019,DOI:10.1111/1348-0421.12754 |
| 录入/更新时间Date | 2021.7.20 |
| 备注Remarks |
