| 中文名称Chinese name
|
HIV HIV-1阴道途径感染人源化hu-BLT小鼠模型 |
| 编号Model number |
|
| 英文名称English name |
HIV HIV-1 intravaginally infected of humanized hu-BLT mouse model |
| 物种Species name |
小鼠
|
| 品系Strain name |
|
| 人类疾病Human diseases |
HIV |
| 传染性Infectivity |
是 |
| 微生物Microorganism |
|
| 类别Category |
HIV感染动物模型 |
| 造模因素Modeling factors |
|
| 制作方法Making method |
The humanized NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ, NSG (hu-NSG) mice were purchased from Jackson Laboratory. Hu-BLT mice were generated by following previously published protocols (8, 9). Those hu-BLT
mice that demonstrated human leukocytes to total leukocytes ratio greater than 50% in peripheral blood, were eligible for challenge with HIV-1 infection. At the end of the experiments, peripheral blood was obtained to determine percentage of human CD4+ cells.Female hu-BLT mice received intraperitoneally 2 mg of methoxyprogesterone 5 days before experiments commenced. Treatment and control mice received intravaginal dose (5 x 10^5 TCID50 in 20 µL) of two HIV transmission/founder (T/F) viruses (WITO.c/2474 and SUMA.c/2821) from acutely infected patients. After 15th week blood draw, treated mice (n=10) received subcutaneously (SubQ) mean dose of 417 mg/kg cARV as TAF+EVG+FTC NP in D5W. Control mice (n=5) received equal volume of D5W SubQ. |
| 生物学特征Biological characteristics |
|
| 医学用途Medical use |
用于HIV致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation |
|
| 病理表现Pathology |
|
| 发病天数Days of onset |
|
| 发病过程Pathogenesis process |
|
| 临床诊断Clinical diagnosis |
|
| 评价模型成功的指标Indicators for evaluating model success |
|
| 保藏单位Preservation unit |
克瑞顿大学@@https://spahp.creighton.edu/ |
| 参考文献Reference |
Long-acting parenteral combination antiretroviral loaded nano-drug delivery system to treat chronic HIV-1 infection: A humanized mouse model study.,PMID:29885378,DOI:10.1016/j.antiviral.2018.06.005 |
| 录入/更新时间Date |
2021.7.20 |
| 备注Remarks |
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