| 中文名称Chinese name
|
SARS PUMC01 滴鼻感染猕猴模型 |
| 编号Model number |
|
| 英文名称English name |
SARS PUMC01 intranasal infected Macaques model |
| 物种Species name |
猴
|
| 品系Strain name |
|
| 人类疾病Human diseases |
SARS |
| 传染性Infectivity |
是 |
| 微生物Microorganism |
|
| 类别Category |
SARS-CoV感染动物模型 |
| 造模因素Modeling factors |
|
| 制作方法Making method |
Briefly, the animals were anesthetized with ketamine (0.5 mL/kg, im). The TCID50 10**−6 (4.0 mL/monkey) of the stock PUMC01 strain virus was sprayed into the nasal cavity of the animals. The peptide and/or peptide mixtures were dissolved as 480 μg/mL of each peptide in 0.9% NaCl and then formulated with an equal volume of Freund’s Complete Adjuvant (FCA) (Sigma, St. Louis, MO, USA) according to the manufacturer’s instructions. The injected dose of vaccine was 0.5 mL/4 kg/monkey (120 μg/4 kg/monkey) given through intramuscular (im) injections at four sites on the arms and legs. |
| 生物学特征Biological characteristics |
|
| 医学用途Medical use |
用于SARS-CoV致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation |
|
| 病理表现Pathology |
|
| 发病天数Days of onset |
|
| 发病过程Pathogenesis process |
|
| 临床诊断Clinical diagnosis |
|
| 评价模型成功的指标Indicators for evaluating model success |
|
| 保藏单位Preservation unit |
清华大学@@https://www.tsinghua.edu.cn |
| 参考文献Reference |
Immunodominant SARS Coronavirus Epitopes in Humans Elicited both Enhancing and Neutralizing Effects on Infection in Non-human Primates.,PMID:27627203,DOI:10.1021/acsinfecdis.6b00006 |
| 录入/更新时间Date |
2021.7.20 |
| 备注Remarks |
|
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