| 制作方法Making method |
In an animal facility, 4–6 h before the planned transplantation of the stem cells, irradiate 6–7 week-old female NOD.Cg-Prkdc scid Il2rgtm1Sug/ JicTac (NOG) mice with 0.75 Gy with a Cs137 source. Prepare to transplant 75,000 CD34+ cells per 200 µL into each mouse. Mice were exposed with 21,400 IUs of RHPA4259 intravaginally, 5,157 IUs IV with RHPA4259, or 3,000 IUs IV with YU2. Details regarding IV exposure of humanized mice are not included in this protocol. HIV infections were successfully treated with a cART regimen delivered through mouse chow.Feed mice with pellets containing a standard cART regimen containing 4,800 mg/kg raltegravir (RAL), 720 mg/kg tenofovir disoproxil fumarate (TDF), and 520 mg/kg emtricitabine (FTC)21 (Table 8). These doses were determined assuming that a mouse weighs 25 g and eats 4 g of chow per day. This corresponds to a daily dose of 768 mg/kg RAL, 2.88 mg/kg TDF, and 83 mg/kg FTC. |
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