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> HIV-1 ADA腹腔途径感染CD34-NSG小鼠模型
| 中文名称Chinese name | HIV-1 ADA腹腔途径感染CD34-NSG小鼠模型 |
|---|---|
| 编号Model number | |
| 英文名称English name | HIV-1 ADA intraperitoneally infected CD34-NSG mouse model |
| 物种Species name | 小鼠 |
| 品系Strain name | |
| 人类疾病Human diseases | HIV |
| 传染性Infectivity | 是 |
| 微生物Microorganism | |
| 类别Category | HIV感染动物模型 |
| 造模因素Modeling factors | |
| 制作方法Making method | Mice with established human hemato-lymphoid reconstitution (∼6–8 months of age) and positive for IL-34 expression were intraperitoneally infected (n =12) with the 104 tissue culture infectious dose50 (TCID50) of macrophage-tropic HIV-1ADA strain.NOG (formally, NOD.Cg-Prkdcscidil2gtmlSug/Jic) and NOD/ShiJcl (NOD) were used. NOG mice were maintained in the CIEA under specific-pathogen-free conditions. NOD mice were purchased from CIEA (Tokyo,Japan). To generate the hIL-34 expressing transgenic NOG mouse (NOG-hIL-34), a linearized DNA vector (pCMV6-XL4) containing human IL-34 cDNA (Origene Technologies, Inc., Rockville, MD, USA) under the control of a CMV promoter, was microinjected into fertilized eggs obtained by mating NOG and NOD mice. |
| 生物学特征Biological characteristics | |
| 医学用途Medical use | 用于HIV致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation | |
| 病理表现Pathology | |
| 发病天数Days of onset | |
| 发病过程Pathogenesis process | |
| 临床诊断Clinical diagnosis | 病毒在血液中复制 |
| 评价模型成功的指标Indicators for evaluating model success | |
| 保藏单位Preservation unit | 内布拉斯加大学医学中心@@https://www.unmc.edu/ |
| 参考文献Reference | Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice,PMID:30832693,DOI:10.1186/s13024-019-0311-y |
| 录入/更新时间Date | 2021.7.20 |
| 备注Remarks |
