| 中文名称Chinese name
|
MERS-CoV hCoV-EMC滴鼻感染BALB/c小鼠模型 |
| 编号Model number |
|
| 英文名称English name |
MERS-CoV hCoV-EMC intranasally infection mouse model |
| 物种Species name |
小鼠
|
| 品系Strain name |
BALB/c小鼠
|
| 人类疾病Human diseases |
MERS |
| 传染性Infectivity |
是 |
| 微生物Microorganism |
|
| 类别Category |
MERS-CoV感染动物模型 |
| 造模因素Modeling factors |
|
| 制作方法Making method |
Female BALB/c mice aged 6–8 weeks were injected intramuscularly (i.m.) with rNTD protein at a dose of 5 or 10 lg with 100 lL of aluminum hydroxide and 10 lg of CpG adjuvant.Mice were anesthetized lightly with isoflurane and transduced intranasally with 2.5×10^8 plaque-forming units (pfu) of Ad5-hDPP4 for rapid generation of a mouse model of MERS-CoV infection as described previously [31]. Five days later,the transduced mice were infected intranasally with MERS-CoV (1×10^5 pfu) in a total volume of 50 μL of DMEM. |
| 生物学特征Biological characteristics |
|
| 医学用途Medical use |
用于MERS致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation |
|
| 病理表现Pathology |
|
| 发病天数Days of onset |
|
| 发病过程Pathogenesis process |
|
| 临床诊断Clinical diagnosis |
|
| 评价模型成功的指标Indicators for evaluating model success |
|
| 保藏单位Preservation unit |
中国疾病预防控制中心@@http://www.chinacdc.cn/en/aboutus/orc_9362/201809/t20180926_194256.html |
| 参考文献Reference |
The recombinant N-terminal domain of spike proteins is a potential vaccine against Middle East respiratory syndrome coronavirus (MERS-CoV) infection.,PMID:27899228,DOI:10.1038/nmicrobiol.2016.288 |
| 录入/更新时间Date |
2021.7.20 |
| 备注Remarks |
|
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