| 中文名称Chinese name
|
SARS-CoV PUMC01 滴鼻感染hACE2 转基因小鼠模型 |
| 编号Model number |
|
| 英文名称English name |
SARS-CoV PUMC01 intranasally infected hACE2 transgenic mice model |
| 物种Species name |
小鼠
|
| 品系Strain name |
|
| 人类疾病Human diseases |
SARS |
| 传染性Infectivity |
是 |
| 微生物Microorganism |
|
| 类别Category |
新型冠状病毒动物模型 |
| 造模因素Modeling factors |
|
| 制作方法Making method |
hACE2 cDNA was cloned as previously described35 and inserted into the pEGFP-N1 plasmid (BD Biosciences) upstream of the SV40 polyA tract, replacing the coding sequence for enhanced green fl uorescence protein. We retrieved the mouse ACE2 promoter (9 kilobases) by digesting bacterial artifi cial chromosome (RP23-75P20) DNA with KpnI and EcoRV and inserted it upstream of the hACE2 coding sequences. After hACE2 was identifi ed in transfected NIH3T3 cells, the plasmid was linearized with KpnI and KasI and purifi ed with phenol.
Fragments carrying the mouse ACE2 promoter driving the hACE2 coding sequence were introduced by microinjection into the pronuclei of fertilized ova from ICR mice. The injected zygotes then were transplanted into the oviducts of pseudopregnant mice. Mice in which the transgene integrated were identifi ed from tail biopsies.
These mice were bred to produce offspring for analysis. |
| 生物学特征Biological characteristics |
|
| 医学用途Medical use |
用于新冠肺炎COVID-19致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation |
|
| 病理表现Pathology |
|
| 发病天数Days of onset |
|
| 发病过程Pathogenesis process |
|
| 临床诊断Clinical diagnosis |
|
| 评价模型成功的指标Indicators for evaluating model success |
|
| 保藏单位Preservation unit |
北京协和医院@@https://www.pumch.cn/en.html |
| 参考文献Reference |
Mice transgenic for human angiotensin-converting enzyme 2 provide a model for SARS coronavirus infection.,PMID:17974127,NA |
| 录入/更新时间Date |
2021.7.20 |
| 备注Remarks |
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