| 制作方法Making method |
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animals were between 10 and 16 weeks. Mice were implanted with either a placebo or 25 mg slowrelease morphine pellet at 24 h prior to infection with EcoHIV. Pellets were generously provided by NIH/NIDA. For long term experiments (>7 days), mice were implanted with either 25 mg or 75 mg morphine pellets using a protocol that would increase released morphine every 7 days to avoid tolerance. Escalating doses in the order used were 1× 25 mg, 1× 75 mg, 2× 25 mg, and 2× 75 mg based on a study that showed 2× 25 mg morphine pellets resulted in higher morphine release than 1× 75 mg pellet, likely due to increased surface area with having two pellets. The first dose given (implant at day 0, exposed 0–7 days) was 25mg slow-release morphine pellet as in short term experiments. The second week (implant at day 7, exposed 7–14 days), mice were pelleted with a 75mg slow-release morphine pellet. The third week (implant at day 14, exposed 14–21 days) mice were pelleted with 2× 25 mg pellets and the final week (implant at day 21, exposed 21–28 days) with 2× 75 mg pellet.Mice were injected in the intraperitoneal cavity with 1 mL of either saline or 1×10^6 pg p24 units as measured by p24 ELISA following manufacturer’s instructions (ZeptoMetrix Corporation). |
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