| 中文名称Chinese name
|
MERS-CoV HCoV-EMC2012滴鼻感染转基因hDPP4-Tg小鼠模型 |
| 编号Model number |
|
| 英文名称English name |
MERS-CoV HCoV-EMC2012 intransally infection transgenic hDPP4-Tg mouse model |
| 物种Species name |
小鼠
|
| 品系Strain name |
|
| 人类疾病Human diseases |
MERS |
| 传染性Infectivity |
是 |
| 微生物Microorganism |
|
| 类别Category |
MERS-CoV感染动物模型 |
| 造模因素Modeling factors |
|
| 制作方法Making method |
Six-week-old female hDPP4-Tg mice27 were maintained in a pathogen-free facility and housed in cages containing sterilized feed and drinking water.r. MERS-CoV (HCoVEMC2012 strain) was propagated and titrated on Vero cells. Following intraperitoneal anesthetization with sodium pentobarbital (5 mg/kg of body weight), mice were treated intravenously (600 μg/kg, i.v.) with a monoclonal Ab (mAb) against mouse C5aR (Hycult Biotech,The Netherlands) or phosphate-buffered saline (PBS) as a sham treatment control. All mice were then intranasally inoculated with MERS-CoV (103.3 50% tissue culture
infectious dose (TCID50)) in 20 μl Dulbecco’s modified Eagle’s medium. |
| 生物学特征Biological characteristics |
|
| 医学用途Medical use |
用于MERS致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation |
|
| 病理表现Pathology |
|
| 发病天数Days of onset |
|
| 发病过程Pathogenesis process |
|
| 临床诊断Clinical diagnosis |
|
| 评价模型成功的指标Indicators for evaluating model success |
|
| 保藏单位Preservation unit |
病原微生物生物安全国家重点实验室@@https://www.natureindex.com/institution-outputs/china/state-key-laboratory-of-pathogen-and-biosecurity-amms/55010c6f140ba079548b4567 |
| 参考文献Reference |
Blockade of the C5a-C5aR axis alleviates lung damage in hDPP4-transgenic mice infected with MERS-CoV.,PMID:29691378,DOI:10.1038/nmicrobiol.2016.285 |
| 录入/更新时间Date |
2021.7.20 |
| 备注Remarks |
|
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