| 中文名称Chinese name
|
MERS-CoV hCoV-EMC/2012气管途径感染猕猴模型 |
| 编号Model number |
|
| 英文名称English name |
MERS-CoV hCoV-EMC/2012 intratracheally infection rhesus macaques model |
| 物种Species name |
猴
|
| 品系Strain name |
|
| 人类疾病Human diseases |
MERS |
| 传染性Infectivity |
是 |
| 微生物Microorganism |
|
| 类别Category |
MERS-CoV感染动物模型 |
| 造模因素Modeling factors |
|
| 制作方法Making method |
High-dose animals were primed with 200 μg rRBD and boosted with 100-μg rRBD admixed with 1 mg of alum adjuvant. Animals received their second immunisation 8 weeks subsequent to their first. To assess long-term immunological responses, the interval between the second and third immunisations was 17 weeks. Each vaccine formulation was administered intramuscularly (i.m.).One week after the final immunisation, animals were transferred to an ABSL-3 laboratory. Following an adaptive phase of 1 week, nine immunised monkeys were anaesthetised and inoculated intratracheally with MERS-CoV of the hCoV-EMC strain at a dosage of 6.5 × 107 TCID50,diluted in 1-mL DMEM. |
| 生物学特征Biological characteristics |
|
| 医学用途Medical use |
用于MERS致病机制研究、疫苗和药物评估。 |
| 临床表现Clinical manifestation |
|
| 病理表现Pathology |
|
| 发病天数Days of onset |
|
| 发病过程Pathogenesis process |
|
| 临床诊断Clinical diagnosis |
|
| 评价模型成功的指标Indicators for evaluating model success |
|
| 保藏单位Preservation unit |
中国疾病预防控制中心@@http://www.chinacdc.cn/en/aboutus/orc_9362/201809/t20180926_194256.html |
| 参考文献Reference |
Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge.,PMID:26629538,DOI:10.1016/j.ebiom.2015.08.031 |
| 录入/更新时间Date |
2021.7.20 |
| 备注Remarks |
|
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